DNA Vectors
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
Amsbio LLC Fibronectin (FN1) Human Gene Knockout Kit (CRISPR)
Fibronectin (FN1) Human Gene Knockout Kit (CRISPR)
IBA LifeSciences StrGate Acptr Vctr pCSG-IBAwt2
pCSG-IBAwt2 is a large expression vector with universal features for transient expression as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Vector Biolabs vascular endothelial growth factor D Adenovirus (Ad-VEGF-D)
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include FGF, PDGF, vascular endothelial growth factor (VEGF), VEGF-B, VEGF-C, VEGF-D (or FIGF), EG-VEGF and placenta growth factor (PlGF). VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.
Eurofins Discoverx PH PROLABEL DETECTN KIT 800DP
The PathHunter® ProLabel Detection Kit is for use with PathHunter Cell Lines expressing ProLabel® (PL) or ProLink™ (PK) expression or cloning vectors. The kit measures the total amount of ProLabel- or ProLink-tagged protein expressed in cells. Functional assays using PathHunter Cell Lines expressing both a ProLabel- or ProLinktagged protein and EA require the PathHunter Detection Kit (93-0001). The assay can be used in both 96-well and 384-well microplate formats. 800 dp (384-well)
BIOHIPPO AAV-hSyn-hChR2(C128S/D156A/T159C)-mCherry-WPRE-hGH-polyA
Recombinant adeno associated virus expressing hChR2(C128S/D156A/T159C) fused to mCherry under the hSyn promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Amsbio LLC Vitronectin (VTN) Human Gene Knockout Kit (CRISPR)
Vitronectin (VTN) Human Gene Knockout Kit (CRISPR)
New England Biolabs, Inc. Lambda DNA (dam-) – 1250 µg
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.
Apexbio Technology LLC Safe DNA Gel Stain 100x1ml
Safe DNA Gel Stain is a fluorescent nucleic acid dye developed for visual detection of DNA or RNA fragments in agarose or polyacrylamide gels during electrophoresis procedures Structurally this reagent binds nucleic acids by intercalating into their double-stranded regions resulting in enhanced fluorescence upon excitation by ultraviolet (UV) or blue light transilluminators It is commonly utilized in molecular biology workflows such as agarose gel electrophoresis polyacrylamide gel electrophoresis and nucleic acid gel purification Designed as a safer alternative to ethidium bromide it reduces mutagenic risks associated with DNA staining and UV exposure thus facilitating downstream recombinant and cloning experiments Safe DNA Gel Stain is provided as a concentrated solution compatible with DNA and RNA visualization in routine laboratory gel-based analyses
Vector Biolabs AAV-RFP-iCre (AAV1) AAV (AAV1-RFP-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.This AAV1 stock expresses an improved Cre (iCre) along with a RFP under a control of the same CMV promoter. The Cre and RFP was separated by a 2A peptides.
Vector Biolabs cAMP responsive element binding protein 1 Adenovirus (Ad-CREB1)
The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, LZIP (also designated CREB3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-4, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A-mediated CREB phosphorylation induces association with a 265 kDa nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator.
Vector Biolabs p16/CDKN2A Adenovirus (Ad-CMV-p16)
p16/CDKN2A is a cyclin-dependent kinase inhibitor that has shown prognostic utility in some human cancers. p16 is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.p16/CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame(ARF) that specifies a protein which is structurally unrelated to the products of the other variants.
Vector Biolabs AAV-DJ-mCherry-iCre AAV (AAVDJ-mCherry-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.This AAV-DJ stock expresses an improved Cre (iCre) and a mCherry driven by one CMV promoter. The iCre and mCherry was separated by a 2A peptides.
Vector Biolabs T-cell factor 4 Adenovirus (Ad-TCF4)
T-cell factor (TCF-1) and lymphoid enhancer-binding factor (for LEF-1, also designated TCF-1?) compose a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. LEF-1, TCF-1, and two other family members, TCF-3 and TCF-4, directly bind to cytosolic beta-catenin and facilitate its translocation to the nucleus, where these complexes induce expression of Wnt target genes.
Vector Biolabs GCaMP6m Adenovirus (Ad-GCaMP6m)
GCaMP is a genetically encoded calcium indicator (GECI) which was created from a fusion of green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. GECI binds Ca2+ and induce a change in fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ fluxes. The advantage of GECI's are that they can be genetically specified for studies in living organisms.The new novel GCaMP6 have increased ΔF/F0 and faster kinetics compared to previous GCaMP3 and GCaMP5G.This adenovirus expresses GCaMP6m.